Journal: IEEE transactions on magnetics

Article Title: Improvement of Reproducibility of Magnetic Moment Detected by a SQUID Magnetometer Through Radial Offset Measurement on a YIG Sphere

doi: 10.1109/tmag.2018.2867477

Figure Lengend Snippet: ϕ-dependences of the magnetic moment for the commercial YIG sphere in one of the custom PTFE containers at 298 K at an applied magnetic field of 397.89 kA · m−1. Each data point was measured three times in succession, and its typical relative standard deviation was 0.02%–0.005%.

Article Snippet: Another sample, a single crystal YIG sphere (Ferrisphere, Inc.) with highly polished surface and a diameter of 1 mm was used for the measurement of the radial offset.

Techniques: Standard Deviation

Journal: IEEE transactions on magnetics

Article Title: Improvement of Reproducibility of Magnetic Moment Detected by a SQUID Magnetometer Through Radial Offset Measurement on a YIG Sphere

doi: 10.1109/tmag.2018.2867477

Figure Lengend Snippet: Radial offset measurement at 298 K using a commercial YIG sphere in the PTFE containers with different r values. The vertical axis is the normalized magnetic moment, i.e., the magnetic moment divided by the minimum magnetic moment at r = 0 mm.

Article Snippet: Another sample, a single crystal YIG sphere (Ferrisphere, Inc.) with highly polished surface and a diameter of 1 mm was used for the measurement of the radial offset.

Techniques:

Journal: IEEE transactions on magnetics

Article Title: Improvement of Reproducibility of Magnetic Moment Detected by a SQUID Magnetometer Through Radial Offset Measurement on a YIG Sphere

doi: 10.1109/tmag.2018.2867477

Figure Lengend Snippet: Correlation of the magnetic moment and the regression factor when the commercial YIG sphere was measured in the PTFE containers. (Representative r values only are shown.)

Article Snippet: Another sample, a single crystal YIG sphere (Ferrisphere, Inc.) with highly polished surface and a diameter of 1 mm was used for the measurement of the radial offset.

Techniques:

Journal: STAR Protocols

Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol

doi: 10.1016/j.xpro.2021.100794

Figure Lengend Snippet: Design of microfluidic devices (A) Device I: single cell encapsulation device for PEGDT-MALDEX hydrogel culturing. (B) Device II: CloneSeq device for single-clone transcriptome barcoding in drops. The numbers indicate the inlets: (1) carrier oil inlet, (2) PEGDT mix, (3) MALDEX mix with cells, (4) outlet for collection, (5) single clone suspension, (6) RT lysis mix, (7) barcoding hydrogel bead.

Article Snippet: For the use of A-i-O software, check the manual of Fluigent TM ( https://www.fluigent.com/download/ ) Microfluidic setup for single cell encapsulation in PEGDT-MALDEX hydrogel sphere (A) Pressure controller.

Techniques: Lysis

Journal: STAR Protocols

Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol

doi: 10.1016/j.xpro.2021.100794

Figure Lengend Snippet: Microfluidic setup for single cell encapsulation in PEGDT-MALDEX hydrogel sphere (A) Pressure controller. Use 2000 mbar pump for carrier oil inlet, 1000 mbar pump for PEGDT and MALDEX inlets. (B) Pressurized reservoir. Use 1.5 mL and 2 mL Safe-Lock tubes for PEGDT and MALDEX mix, respectively and use a 15 mL tube for 2% HFE-7500 oil. (C) Cell mixer. The yellow square shows a zoom in on the MALDEX mix. The tube is surrounded by an ice stand, and the cell mixer is working outside of the tube towards the bottom of the tube, to make the magnetic stirring bar agitate the cells and prevent sedimentation. (D) Flow sensor. Use Flow unit size M for all three inlets. (E) Microfluidic device for single cell encapsulation in PEGDT-MALDEX hydrogel sphere. A zoom in on the design, indicating the position of each inlet: (1) 2% HFE-7500 oil, (2) PEGDT mix, (3) MALDEX mix and (4) outlet for collection. (F) Collection tube. Use a 2 mL tube, placed in a cooling block. (G) Control software for the microscope, zoom in on the droplet-generation junction. (H) A-i-O software for flow control.

Article Snippet: For the use of A-i-O software, check the manual of Fluigent TM ( https://www.fluigent.com/download/ ) Microfluidic setup for single cell encapsulation in PEGDT-MALDEX hydrogel sphere (A) Pressure controller.

Techniques: Sedimentation, Blocking Assay, Software, Microscopy

Journal: STAR Protocols

Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol

doi: 10.1016/j.xpro.2021.100794

Figure Lengend Snippet: Single cell encapsulation in PEGCT-MALDEX hydrogel sphere Red arrows indicate boundary where PEGDT and MALDEX meet. Scale bar: 50 μm.

Article Snippet: For the use of A-i-O software, check the manual of Fluigent TM ( https://www.fluigent.com/download/ ) Microfluidic setup for single cell encapsulation in PEGDT-MALDEX hydrogel sphere (A) Pressure controller.

Techniques:

Journal: STAR Protocols

Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol

doi: 10.1016/j.xpro.2021.100794

Figure Lengend Snippet: Microscope photo of ESC in PEGDT-MALDEX hydrogel after encapsulation The big transparent spheres are PEGDT-MALDEX hydrogel spheres. They are about the size of 70 μm diameter. Red arrows indicate single cells in hydrogel spheres. Yellow arrows indicate oil drops. Scale bar: 50 μm.

Article Snippet: For the use of A-i-O software, check the manual of Fluigent TM ( https://www.fluigent.com/download/ ) Microfluidic setup for single cell encapsulation in PEGDT-MALDEX hydrogel sphere (A) Pressure controller.

Techniques: Microscopy

Journal: STAR Protocols

Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol

doi: 10.1016/j.xpro.2021.100794

Figure Lengend Snippet: ESCs in PEGDT-MALDEX hydrogel Left picture shows the cells after encapsulation into hydrogel, right picture shows clones developed after 3 days of culture. The round-shaped cells are clones well defined in hydrogels, and cells with dendrites are cells that escaped from the hydrogel and grew on the tissue culture plate. Scale bars: 50 μm.

Article Snippet: For the use of A-i-O software, check the manual of Fluigent TM ( https://www.fluigent.com/download/ ) Microfluidic setup for single cell encapsulation in PEGDT-MALDEX hydrogel sphere (A) Pressure controller.

Techniques: Clone Assay